Studies of growth control and gene expression in normal and neoplastic B-lymphoid cells
Studies of growth control and gene expression in normal and neoplastic B-lymphoi
Prosjektet er en del av et flerårig prosjekt som involverer flere postdoktorer og stipendiater. Midlene har vært benyttet til delfinansiering av en postdoktor og til driftsmidler. Hovedfokus er karakterisering av genendringer og genekspresjon i maligne lymfomer og funksjonelle studier av signalveier.
We are one of 7 groups that constitute the newly established Centre for Cancer Biomedicine at the Institute and we are part of the lymphoma milieu at our hospital. Studies of genetic aberrations and changes in gene expression by use of microarray analyses represents a central part of our research activity. We are involved in a broad international collaboration led from NCI regarding a broad molecular characterization of Non Hodgkin’s lymphoma by use of gene expression profiling. Three distinct subgroups of diffuse large B cell lymphoma have been identified
and these have different prognosis and pathogenesis. For several of the major lymphoma subtypes, we have found that expression of different sets of genes are strongly correlated to prognosis. We have now also entered a prospecive diagnostic study to test the applicability of a specially designed chip in the routine diagnostics of
lymphoma (1/8 centres world-wide). In 2007 we published an article regarding molecular profiling of early human B lymphopoiesis (Hystad et al).
We also use high throughput analyses to study genetic changes in relation to progression of follicular lymphoma. In addition, we collaborate with the lymphoma milieu regarding biological and molecular studies in relation to clinical studies. Studies to explore the role of aberrant signalling pathways represent another main research activity. We have established good in vitro systems to study functional responses in normal and neoplastic B cells. We now focus on the role of members of the TGF-beta/BMP (bone morphogenic protein) family and downstream target genes (including Id-proteins). We have published several articles regarding the role of BMPs in normal B and T lymphocytes. We also have been involved in studies of Wnt signalling in precursor B cells.
Wnt3A activates canonical Wnt signalling in acute lymphoblastic leukaemia (ALL) cells and inhibits the proliferation of B-ALL cell lines.
Br J Haematol 2007 Feb;136(3):400-13. Epub 2006 des 8
Point mutations and genomic deletions in CCND1 create stable truncated cyclin D1 mRNAs that are associated with increased proliferation rate and shorter survival.
Blood 2007 Jun;109(11):4599-606. Epub 2007 feb 13
Aberrant immunoglobulin class switch recombination and switch translocations in activated B cell-like diffuse large B cell lymphoma.
J Exp Med 2007 Mar;204(3):633-43. Epub 2007 mar 12
Characterization of early stages of human B cell development by gene expression profiling.
J Immunol 2007 Sep;179(6):3662-71.
Inhibitory effects and target genes of bone morphogenetic protein 6 in Jurkat TAg cells.
Eur J Immunol 2007 Oct;37(10):2937-48.
Characterization of signaling pathways in normal and malignant hematopoietic cells by microarray technologies
- april 2007
- Erlend B. Smeland
Studies of tumor associated antigens and BMP-6 signalling in normal and neoplastic B cells
- februar 2007
- Erlend B. Smeland