Establishment of an obesity biobank for inflammation studies
Establishment of an obesity bibank for inflammation studies
Obesity is associated with increased inflammation involving interplay between adipose tissue and the immune system, but the mechanisms are only partly understood. The aim of this project is to establish an obesity biobank for inflammation studies for increased understanding of obesity related processes involved in disease development.
Adipose tissue plays a role in energy storage and in hormonal and inflammatory processes that might contribute to development of inflammatory diseases like cardiovascular disease, metabolic syndrome, diabetes and preeclampsia. New insight is expected gained from study of inflammatory and immunological mechanisms related to adipose tissue and obesity. In this study biological material from lean and obese persons collected from hernia operations, weight loss surgery and liposuction will be collected to an obesity biobank. The collected materials will be analyzed by immunological, histological and functional methods. The research is expected to gain knowledge on underlying molecular and genetic mechanisms and potentially identify new biomarkers and therapies related to obesity and the contribution of obesity and adipose tissue to other diseases. The initial phase of this project has been focused on establishment of detailed protocols for collection of biologic samples of high quality from the three patient groups; lean and obese persons undergoing hernia operation, weight loss surgery and liposuction at three different hospital departments. All protocols are in place for collection of clinical information and biological samples from the patients and further processing of biological samples for storage. All other documentation is also in place; project information and consent forms for the participants and ethical evaluation of the biobank collection and research project. Application for ethical approval was submitted in December 2015 and the biobank collection will start as soon as ethical approval has been given. Protocols for isolation of adipocytes from adipose tissue samples after liposuction has been tested and improved to prepare for optimal cell isolation on patient samples after start of biobank collection.