Establishment of an obesity biobank for inflammation studies
Prosjekt
- Prosjektnummer
- 46083100
- Ansvarlig person
- Ann-Charlotte Iversen
- Institusjon
- NTNU, IKOM
- Prosjektkategori
- Korttidsprosjekt 2015
- Helsekategori
- Metabolic and Endocrine
- Forskningsaktivitet
- 4. Detection and Diagnosis
Rapporter
Obesity is associated with increased inflammation involving an interplay between adipose tissue and the immune system, but the mechanisms are only partly understood. The aim of this project has been to establish an obesity biobank for inflammation studies for increased understanding of obesity related processes involved in disease development.
Adipose tissue plays a role in energy storage and hormonal and inflammatory processes that might contribute to development of inflammatory diseases like cardiovascular disease, metabolic syndrome, diabetes and preeclampsia. New insight is expected gained from study of inflammatory and immunological mechanisms related to adipose tissue and obesity.
In this study, an obesity biobank has been established in close collaboration between NTNU, several departments at St. Olavs Hospital and Aleris. Clinical information and biological materials from lean and obese persons from gall bladder operations, bariatric surgery and liposuction has been collected to an obesity biobank. The planned recruitment of 100 individuals has been completed.
The biological materials, including sampling of subcutaneous and visceral adipose tissue, blood and urine, has been quality controlled and confirmed of high quality. Methods for immunological, histological, advanced imaging, and in vitro culture studies of adipose tissue have been established as planned.
Immunohistochemical staining for expression of inflammatory markers in adipose tissue has revealed potent inflammatory properties for macrophages. Cultured adipose tissue explants showed cytokine response to inflammatory activation in functional support of the tissue expression analyses. Immune activation analysis in subcutaneous and visceral fat comparing lean and obese individuals points to promising differences.
This project has established a new obesity biobank collection and relevant methods for translational studies as planned. Initial analyses have revealed potent inflammatory processes at play in subcutaneous and visceral adipose tissue, with macrophages as central cellular players. The project has been disseminated in regional hospitals and NTNU, at an international conference, and in the national TV Programme NRK1 “Viten og vilje”. The analyses will be completed as part of an ongoing follow-up project funded by FFU. The planned further research is made possible through the establishment of this valuable obesity biobank. It provides the possibility of gaining new knowledge on underlying obesity-related disease mechanisms that can potentially identify new biomarkers and therapies, and reveal how obesity and adipose tissue contributes to other diseases.
Etableringen av en fedmebiobank av høy kvalitet har gitt en rekke nye samarbeid på tvers av avdelinger og mellom Orkdal sykehus, St Olavs Hospital, Aleris og NTNU. Samarbeidet er basert på en sterk interaksjon mellom molekylære og kliniske miljøer som har betydning for videre forskning og biobank-innsamling. Prosjektet har således lagt til rette for ekte translasjonell forskning som har potensiale for å avsløre nye sykdomsprosesser som identifiserer individer med fettvevs-dysfunksjon og som kan gi grunnleggende forståelse av hvordan overvekt bidrar til utvikling av andre sykdommer.
Obesity is associated with increased inflammation involving an interplay between adipose tissue and the immune system, but the mechanisms are only partly understood. The aim of this project is to establish an obesity biobank for inflammation studies for increased understanding of obesity related processes involved in disease development.Adipose tissue plays a role in energy storage and hormonal and inflammatory processes that might contribute to development of inflammatory diseases like cardiovascular disease, metabolic syndrome, diabetes and preeclampsia. New insight is expected gained from study of inflammatory and immunological mechanisms related to adipose tissue and obesity. In this study, biological material from lean and obese persons collected from gall bladder operations, bariatric surgery and liposuction is being collected to an obesity biobank. The collected materials will be analysed by immunological, histological and functional methods. The research is expected to gain knowledge on underlying molecular and genetic mechanisms and potentially identify new biomarkers and therapies related to obesity and the contribution of obesity and adipose tissue to other diseases.
The project has established all approvals, protocols, logistics and collaborations required for recruitment, biobank collection, sample processing and clinical validation. During 2017, the biobank collection of subcutaneous and visceral adipose tissue and blood and urine samples has been established and more than half of the planned patients have been recruited and their samples and clinical information processed. The collected samples have been controlled and are of high quality. The patient sampling at one year after surgical procedures has started.
Protocols for Immunohistochemical and advances image analysis of adipose tissue are in place and staining for inflammatory markers have revealed potent inflammatory properties for adipose tissue macrophages. Immune activation analysis in subcutaneous and visceral fat comparing lean and obese individuals are ongoing. Cultured adipose tissue explants from liposuction have revealed cytokine response to inflammatory activation in support of the tissue expression analyses. The results will be presented at the Toll 2018 conference in Portugal and a manuscript is in preparation.
Obesity is associated with increased inflammation involving interplay between adipose tissue and the immune system, but the mechanisms are only partly understood. The aim of this project is to establish an obesity biobank for inflammation studies for increased understanding of obesity related processes involved in disease development.Adipose tissue plays a role in energy storage and in hormonal and inflammatory processes that might contribute to development of inflammatory diseases like cardiovascular disease, metabolic syndrome, diabetes and preeclampsia. New insight is expected gained from study of inflammatory and immunological mechanisms related to adipose tissue and obesity. In this study biological material from lean and obese persons collected from hernia operations, weight loss surgery and liposuction will be collected to an obesity biobank. The collected materials will be analyzed by immunological, histological and functional methods. The research is expected to gain knowledge on underlying molecular and genetic mechanisms and potentially identify new biomarkers and therapies related to obesity and the contribution of obesity and adipose tissue to other diseases.
The initial phase of this project has been focused on establishment of detailed protocols for collection of biologic samples of high quality from the three patient groups; lean and obese persons undergoing hernia operation, weight loss surgery and liposuction at three different hospital departments. All protocols are in place for collection of clinical information and biological samples from the patients and further processing of biological samples for storage. All other documentation is also in place; project information and consent forms for the participants and ethical evaluation of the biobank collection and research project. Application for ethical approval was submitted in December 2015 and the biobank collection will start as soon as ethical approval has been given. Protocols for isolation of adipocytes from adipose tissue samples after liposuction has been tested and improved to prepare for optimal cell isolation on patient samples after start of biobank collection.
Deltagere
- Ivan Pavlovic Prosjektdeltaker
- Lobke Marijn Gierman Postdoktorstipendiat
- Hege Tevik Bjøru Prosjektdeltaker
- Anne Mari Aukan Rokstad Prosjektdeltaker
- Bård Eirik Kulseng Prosjektdeltaker
- Ann-Charlotte Iversen Prosjektleder
- Gjermund Johnsen Prosjektdeltaker
- Ronald Mårvik Prosjektdeltaker
eRapport er utarbeidet av Sølvi Lerfald og Reidar Thorstensen, Regionalt kompetansesenter for klinisk forskning, Helse Vest RHF, og videreutvikles av de fire RHF-ene i fellesskap, med støtte fra Helse Vest IKT
Alle henvendelser rettes til Helse Midt-Norge RHF - Samarbeidsorganet og FFU