HSØ Core Facility for Super-Resolution Microscopy
Super-resolution microscopy is a new form of light microscopy with greatly enhanced optical resolution. Due to the diffraction of light emitted by fluorophores, the maximal resolution achievable by conventional fluorescence microscopy (including confocal microscopy) is limited to 200-300 nm, leaving many biological sub-structures and organelles unresolved. Super-resolution microscopes, on the other hand, provide an image resolution of biological speciments between 10 and 100 nm (with a theoretical lower threshold of 1 nm). The enhanced resolution achieved by these new microscopy technologies is expected to greatly impact our understanding of biological processes as a large number of biological structures structures, including chromosomes, membrane embedded organelles, protein filaments, bacterial sub-compartments and viruses can now be imaged at molecular resolution. Super-resolution microscopes are very expensive and require highly trained personnel that can assist with calibration, microscopy and post-microscopy processing. For these reasons, super-resolution microscopy has been offered as a core facility service. The facility have been divided into two separate nodes that have provided services and expertise on different but complementary super-resolution technologies, including gated stimulated emission depletion (gSTED) microscopy, structured illumination microscopy (SIM), and stochastic optical reconstruction microscopy (STORM). The first node have been localized at the Department of Medical Biochemistry, Clinic for Investigation and Diagnostics, Rikshospitalet, while the other node have been located at Institute for Cancer Research at Clinic for Cancer Medicine, Surgery and Transplantation, the Norwegian Radium Hospital. The facility have assisted researchers in the Oslo region with sample preparation, instrumentation, data processing and data interpretation. Notably, the respective laboratories are the first in Norway to acquire super-resolution microscopes.
The group leaders at the two core facility nodes are the first in Norway to have acquired super-resolution microscopes. The personnel at the two core facility nodes have created an internationally competitive super-resolution imaging service that provides state of the art instrumentation and highly qualified hands-on supervision for core facility users. From January 2015 this core facility will be a part of HSØ core facility for advanced light microscopy.
Photoactivation approaches reveal a role for Rab11 in FGFR4 recycling and signalling.
Traffic 2014 Jun;15(6):665-83. Epub 2014 mar 31
Monitoring phosphatidylinositol 3-phosphate in multivesicular endosome biogenesis.
Methods Enzymol 2014;534():3-23.
Promyelocytic leukemia bodies tether to early endosomes during mitosis.
Cell Cycle 2014;13(11):1749-55. Epub 2014 mar 26
Dynamic Escherichia coli SeqA complexes organize the newly replicated DNA at a considerable distance from the replisome
Nucleic Acids Research, In Press