Finansiering av laboratoriekostnader
Degradation, formation and redistribution of drugs post mortem
Status: innsamlingen begynte i 2006. Vi har nå 8 000 prøver fra 490 rettslige obduksjoner. Vi tilsetter nå en PhD kandidat i prosjektet. Prøvene er lagret i frysere. Kliniske data og prøvedata kan akkurat nå begynne å bli registrert i databasen til Biobanken. Omdisponering av midlene til innkjøp av fryser ble godkjent av prodekanus forskning DMF.
In forensic autopsy cases, results from qualitative and quantitative analysis of alcohols, medicinal and illicit substances in body fluids are often of pivotal importance when deciding the underlying, immediate and contributory causes of death. The correct interpretation of such findings may in turn have important criminal and civil legal implications.
The routine therapeutic and toxicological monitoring in medical practice is usually based on analyses in serum samples from patients. However, several biological processes that occur after death may affect post mortem measurements. Often, drugs distribute unevenly in whole blood, and serum to blood ratios may sometimes approach a factor of 10. In addition, drugs may be subjected to post mortem biological degradation/metabolism. Some substances, such as ethanol and gamma-hydroxybutyrate (GHB), may be formed in clinically significant levels after death. Ingested drugs, present as “reservoirs” in the stomach or small intestine, may seep into surrounding tissues after death, and drugs which are highly bound to tissue components may become unbound and redistribute as the autolytic process commences. Our information about the qualitative and quantitative extent of such processes is based on case studies and experimental models in animals, both of which may have limited applicability to actual cases. There is also a need to better understand the effect of storage condition and storage time on the analytical outcomes.
In forensic autopsy cases, analyses of alcohols, medicinal and illicit substances in body fluids are often of pivotal importance. Several biological processes that occur after death may affect post mortem measurements. Specimens from 490 cases have so far been collected. We propose to undertake a detailed analysis of samples from this large collection of forensic cases to enhance the knowledge regarding the fate of drugs in deceased subjects.
Using state of the art methodology and an expanding biobank encompassing all cases analysed in our laboratories through the last few years, we propose to undertake a detailed qualitative and quantitative analyses of series of selected forensic cases. In particular, the investigation will be focused on:
1. the toxicity profile and putative redistribution of widely used sleeping drugs of the z-hypnotic class in overdose cases
2. the toxicity profile and putative redistribution of the most recent psychotropic drugs of the neuroleptic and antidepressant classes in overdose cases
3. the redistribution and metabolic fate of opioids in overdose cases
4. the formation and distribution of GHB in non-GHB-related deaths