Novel viruses in childhood respiratory tract infections
Prosjekt
- Prosjektnummer
- 46056618
- Ansvarlig person
- Jørn Klein
- Institusjon
- NTNU, LBK
- Prosjektkategori
- Forskerstipend 2012
- Helsekategori
- Respiratory
- Forskningsaktivitet
- 4. Detection and Diagnosis
Rapporter
Acute respiratory tract infections (RTI) are leading causes of childhood morbidity and mortality worldwide, resulting in nearly 2 million deaths annually. Even in the Western World, RTI contribute more to the disease burden and health care costs than any other infectious disease.Despite its abundance and potential severity, we still have limited information about causes, patophysiology and the best ways to treat various RTI.
In children, more than three-fourth of all RTI may be caused by viruses, and today over 200 various respiratory viruses are known. Nevertheless, in recent studies on childhood RTI, more than one-fourth of respiratory samples are virus negative, despite the use of comprehensive diagnostic approaches. Thus, it is likely that more respiratory viruses do exist, but they may escape detection from present diagnostic tests.
The present project is based on the use of the samples from patients and controls which are negative for all PCR-tests, and negative by viral and bacterial cultures. We apply metagenomics analysis to identify novel viruses and virus variants. This is a new method for the detection of previously unknown viruses, based on pyro-sequencing platforms and bioinformatical analysis. This method is able to detect very small amounts of genetic material directly from the sample by sequencing large amounts of DNA/cDNA fragments.
Based on these results, specific PCR tests are and will be developed, and together with my collaborators in the project “Luftveisinfeksjoner hos barn” we will are studying the occurrence and clinical manifestations in the entire sample collection.
In 2013 we have
• Performed bioinformatics analysis of achieved data
• Detected the unusal abscence of different Herpesviruses in samples from children with resperiatory disease
• Developed an pan-herpes PCR for further analysis
• Tested a new pan- hMNPV PCR based on Primer Probe Energy Transfer technology
• Established a new virology course to inform and atract students to the aims of the project
• Supervision of 1 MSc-student in Molecular Medicine working in this project
Acute respiratory tract infections (RTI) are leading causes of childhood morbidity and mortality worldwide, resulting in nearly 2 million deaths annually. Even in the Western World, RTI contribute more to the disease burden and health care costs than any other infectious disease.Despite its abundance and potential severity, we still have limited information about causes, patophysiology and the best ways to treat various RTI.
In children, more than three-fourth of all RTI may be caused by viruses, and today over 200 various respiratory viruses are known. Nevertheless, in recent studies on childhood RTI, more than one-fourth of respiratory samples are virus negative, despite the use of comprehensive diagnostic approaches. Thus, it is likely that more respiratory viruses do exist, but they may escape detection from present diagnostic tests.
The present project is based on the use of the samples from patients and controls which are negative for all PCR-tests, and negative by viral and bacterial cultures. We apply metagenomics analysis to identify novel viruses and virus variants. This is a new method for the detection of previously unknown viruses, based on pyro-sequencing platforms and bioinformatical analysis. This method is able to detect very small amounts of genetic material directly from the sample by sequencing large amounts of DNA/cDNA fragments.
Based on these results, specific PCR tests are and will be developed, and together with my collaborators in the project “Luftveisinfeksjoner hos barn” we will are studying the occurrence and clinical manifestations in the entire sample collection.
In 2012 we have
• Established the lab and procedures of biosafety
• Processed together with the University of Oslo the first samples for pyro-sequencing
• Developed a bioinformatics analysis pipeline and analyzed the first data
• Developed a new pan- hMNPV PCR based on Primer Probe Energy Transfer technology
• Established a microarray assay for the detection of all known virus
• Supervision of 1 MSc-student in Molecular Medicine working in this project
Vitenskapelige artikler
Klein J
Emerging viral disease
Rotary Trondheim
Klein J
Emerging airway viruses
Internundervisning AMM, St.Olav's Hospital
Klein J.
Biosafety considerations by working with airborne infections
European Biosafety Association (EBSA) conference, Bassel, 2013
Klein J
Detection of novel virus
Internundervisning AMM, St.Olav's Hospital
Klein J
Microarray technology for virus detection
Charite Hospital Berlin
Klein J
Luftveisinfeksjoner hos barn
Rotary Trondheim
Deltagere
- Henrik Døllner Prosjektdeltaker
- Svein Arne Nordbø Prosjektdeltaker
eRapport er utarbeidet av Sølvi Lerfald og Reidar Thorstensen, Regionalt kompetansesenter for klinisk forskning, Helse Vest RHF, og videreutvikles av de fire RHF-ene i fellesskap, med støtte fra Helse Vest IKT
Alle henvendelser rettes til Helse Midt-Norge RHF - Samarbeidsorganet og FFU